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KMID : 0380219960290010088
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Journal of Biochemistry and Molecular Biology
1996 Volume.29 No. 1 p.88 ~ p.91
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Fnr, NarL and NarP Regulation and Time Course Expression of Escherichia coli aeg-46.5 Gene
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Ahn Ju-Hyuk
Choe Mu-Hyeon
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Abstract
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The anaerobically expressed gene aeg-46.5, which had been identified by the operon fusion technique with a hybrid bacteriophage of λ and Mu, λplacMu53, was studied for its expression pattern and growth. The expression of aeg-46.5 was studied in the wild-type cell and mutant cells that have mutation (s) in the control gene of anaerobic respiration (fnr) and nitrate response (narL and narP). The β-galactosidase reporter gene showed maximum expression in narL host after two hours of aerobic to anaerobic switch in M9-Glc-nitrate medium. Both 40 mM and 100 mM concentrations of nitrate ion in the medium had little effect on expression level. We propose that aeg-46.5 is subject to multiple regulations of anaerobic activation by Fnr, nitrate activation by NarP and repression mediated by NarL.
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KEYWORD
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anaerobically expressed gene, aeg-46.5, nitrate induction, NarL, NarP
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